flow rate sensor 4274 Search Results


ap 24534  (Bio-Techne corporation)
90
Bio-Techne corporation ap 24534
Ap 24534, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+rate+sensor+4274/AP+24534/custom%404274%4034515301
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ap 24534 - by Bioz Stars, 2026-11
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mir-4274 inhibitors  (Shanghai GenePharma)
90
Shanghai GenePharma mir-4274 inhibitors
MiRNAs differentially regulated the allele variants of GWAS lead SNPs. (A–E) Schematic diagram of the miR-153-3p, miR-374b-3p, <t>miR-4274,</t> miR-572 and miR-2964a-5p binding sites on CCDC170 . (F) The overexpression of miR-153-3p significantly suppressed rs6932603-T allele levels in U2OS cells and 293T cells. (G,H) The overexpression of miR-374b-3p and miR-4274 significantly suppressed rs3757322-T allele levels in U2OS cells and 293T cells. (I,J) The overexpression of miR-572 and miR-2964a-5p significantly suppressed rs3748806-G allele levels in U2OS cells and 293T cells. (K) Knockdown of miR-153-3p significantly upregulated rs6932603-T allele levels in U2OS cells and 293T cells. (L,M) Knockdown of miR-374b-3p and miR-4274 significantly upregulated rs3757322-T allele levels in U2OS cells and 293T cells. (N,O) Knockdown of miR-572 and miR-2964a-5p significantly upregulated rs3748806-G allele levels in U2OS cells and 293T cells. (P) Overexpression of these five miRNAs can reduce the expression of CCDC170 at the protein level, while inhibition of this five miRNAs can increase the expression of CCDC170 . The data are from three independent transfection experiments, with assays performed in triplicate ( n =6). Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars show the standard deviation for six technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. ** P <0.01, *** P <0.001.
Mir 4274 Inhibitors, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+rate+sensor+4274/mir+4274+inhibitors/pmc08061906-93-8-40
Average 90 stars, based on 1 article reviews
mir-4274 inhibitors - by Bioz Stars, 2026-11
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m-scm-5k  (JenKem Inc)
90
JenKem Inc m-scm-5k
MiRNAs differentially regulated the allele variants of GWAS lead SNPs. (A–E) Schematic diagram of the miR-153-3p, miR-374b-3p, <t>miR-4274,</t> miR-572 and miR-2964a-5p binding sites on CCDC170 . (F) The overexpression of miR-153-3p significantly suppressed rs6932603-T allele levels in U2OS cells and 293T cells. (G,H) The overexpression of miR-374b-3p and miR-4274 significantly suppressed rs3757322-T allele levels in U2OS cells and 293T cells. (I,J) The overexpression of miR-572 and miR-2964a-5p significantly suppressed rs3748806-G allele levels in U2OS cells and 293T cells. (K) Knockdown of miR-153-3p significantly upregulated rs6932603-T allele levels in U2OS cells and 293T cells. (L,M) Knockdown of miR-374b-3p and miR-4274 significantly upregulated rs3757322-T allele levels in U2OS cells and 293T cells. (N,O) Knockdown of miR-572 and miR-2964a-5p significantly upregulated rs3748806-G allele levels in U2OS cells and 293T cells. (P) Overexpression of these five miRNAs can reduce the expression of CCDC170 at the protein level, while inhibition of this five miRNAs can increase the expression of CCDC170 . The data are from three independent transfection experiments, with assays performed in triplicate ( n =6). Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars show the standard deviation for six technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. ** P <0.01, *** P <0.001.
M Scm 5k, supplied by JenKem Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+rate+sensor+4274/m+scm+5k/us11793881-1933-19-26
Average 90 stars, based on 1 article reviews
m-scm-5k - by Bioz Stars, 2026-11
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genentech u.s. pat  (Genentech inc)
90
Genentech inc genentech u.s. pat
MiRNAs differentially regulated the allele variants of GWAS lead SNPs. (A–E) Schematic diagram of the miR-153-3p, miR-374b-3p, <t>miR-4274,</t> miR-572 and miR-2964a-5p binding sites on CCDC170 . (F) The overexpression of miR-153-3p significantly suppressed rs6932603-T allele levels in U2OS cells and 293T cells. (G,H) The overexpression of miR-374b-3p and miR-4274 significantly suppressed rs3757322-T allele levels in U2OS cells and 293T cells. (I,J) The overexpression of miR-572 and miR-2964a-5p significantly suppressed rs3748806-G allele levels in U2OS cells and 293T cells. (K) Knockdown of miR-153-3p significantly upregulated rs6932603-T allele levels in U2OS cells and 293T cells. (L,M) Knockdown of miR-374b-3p and miR-4274 significantly upregulated rs3757322-T allele levels in U2OS cells and 293T cells. (N,O) Knockdown of miR-572 and miR-2964a-5p significantly upregulated rs3748806-G allele levels in U2OS cells and 293T cells. (P) Overexpression of these five miRNAs can reduce the expression of CCDC170 at the protein level, while inhibition of this five miRNAs can increase the expression of CCDC170 . The data are from three independent transfection experiments, with assays performed in triplicate ( n =6). Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars show the standard deviation for six technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. ** P <0.01, *** P <0.001.
Genentech U.S. Pat, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+rate+sensor+4274/genentech+u+s++pat/us07173031-60-6-5
Average 90 stars, based on 1 article reviews
genentech u.s. pat - by Bioz Stars, 2026-11
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u.s. pat  (Genentech inc)
90
Genentech inc u.s. pat
MiRNAs differentially regulated the allele variants of GWAS lead SNPs. (A–E) Schematic diagram of the miR-153-3p, miR-374b-3p, <t>miR-4274,</t> miR-572 and miR-2964a-5p binding sites on CCDC170 . (F) The overexpression of miR-153-3p significantly suppressed rs6932603-T allele levels in U2OS cells and 293T cells. (G,H) The overexpression of miR-374b-3p and miR-4274 significantly suppressed rs3757322-T allele levels in U2OS cells and 293T cells. (I,J) The overexpression of miR-572 and miR-2964a-5p significantly suppressed rs3748806-G allele levels in U2OS cells and 293T cells. (K) Knockdown of miR-153-3p significantly upregulated rs6932603-T allele levels in U2OS cells and 293T cells. (L,M) Knockdown of miR-374b-3p and miR-4274 significantly upregulated rs3757322-T allele levels in U2OS cells and 293T cells. (N,O) Knockdown of miR-572 and miR-2964a-5p significantly upregulated rs3748806-G allele levels in U2OS cells and 293T cells. (P) Overexpression of these five miRNAs can reduce the expression of CCDC170 at the protein level, while inhibition of this five miRNAs can increase the expression of CCDC170 . The data are from three independent transfection experiments, with assays performed in triplicate ( n =6). Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars show the standard deviation for six technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. ** P <0.01, *** P <0.001.
U.S. Pat, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+rate+sensor+4274/u+s++pat/us07732613-57-6-5
Average 90 stars, based on 1 article reviews
u.s. pat - by Bioz Stars, 2026-11
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4% gelatin gel (w/v) cat# 4274.1  (Carl Roth GmbH)
90
Carl Roth GmbH 4% gelatin gel (w/v) cat# 4274.1
MiRNAs differentially regulated the allele variants of GWAS lead SNPs. (A–E) Schematic diagram of the miR-153-3p, miR-374b-3p, <t>miR-4274,</t> miR-572 and miR-2964a-5p binding sites on CCDC170 . (F) The overexpression of miR-153-3p significantly suppressed rs6932603-T allele levels in U2OS cells and 293T cells. (G,H) The overexpression of miR-374b-3p and miR-4274 significantly suppressed rs3757322-T allele levels in U2OS cells and 293T cells. (I,J) The overexpression of miR-572 and miR-2964a-5p significantly suppressed rs3748806-G allele levels in U2OS cells and 293T cells. (K) Knockdown of miR-153-3p significantly upregulated rs6932603-T allele levels in U2OS cells and 293T cells. (L,M) Knockdown of miR-374b-3p and miR-4274 significantly upregulated rs3757322-T allele levels in U2OS cells and 293T cells. (N,O) Knockdown of miR-572 and miR-2964a-5p significantly upregulated rs3748806-G allele levels in U2OS cells and 293T cells. (P) Overexpression of these five miRNAs can reduce the expression of CCDC170 at the protein level, while inhibition of this five miRNAs can increase the expression of CCDC170 . The data are from three independent transfection experiments, with assays performed in triplicate ( n =6). Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars show the standard deviation for six technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. ** P <0.01, *** P <0.001.
4% Gelatin Gel (W/V) Cat# 4274.1, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+rate+sensor+4274/4++gelatin+gel++w+v++Cat++4274+1/pm38740835-288-1-6
Average 90 stars, based on 1 article reviews
4% gelatin gel (w/v) cat# 4274.1 - by Bioz Stars, 2026-11
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embocept s microspheres  (PharmaCept GmbH)
90
PharmaCept GmbH embocept s microspheres
MiRNAs differentially regulated the allele variants of GWAS lead SNPs. (A–E) Schematic diagram of the miR-153-3p, miR-374b-3p, <t>miR-4274,</t> miR-572 and miR-2964a-5p binding sites on CCDC170 . (F) The overexpression of miR-153-3p significantly suppressed rs6932603-T allele levels in U2OS cells and 293T cells. (G,H) The overexpression of miR-374b-3p and miR-4274 significantly suppressed rs3757322-T allele levels in U2OS cells and 293T cells. (I,J) The overexpression of miR-572 and miR-2964a-5p significantly suppressed rs3748806-G allele levels in U2OS cells and 293T cells. (K) Knockdown of miR-153-3p significantly upregulated rs6932603-T allele levels in U2OS cells and 293T cells. (L,M) Knockdown of miR-374b-3p and miR-4274 significantly upregulated rs3757322-T allele levels in U2OS cells and 293T cells. (N,O) Knockdown of miR-572 and miR-2964a-5p significantly upregulated rs3748806-G allele levels in U2OS cells and 293T cells. (P) Overexpression of these five miRNAs can reduce the expression of CCDC170 at the protein level, while inhibition of this five miRNAs can increase the expression of CCDC170 . The data are from three independent transfection experiments, with assays performed in triplicate ( n =6). Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars show the standard deviation for six technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. ** P <0.01, *** P <0.001.
Embocept S Microspheres, supplied by PharmaCept GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+rate+sensor+4274/embocept+s+microspheres/pm37686549-87-23-26
Average 90 stars, based on 1 article reviews
embocept s microspheres - by Bioz Stars, 2026-11
90/100 stars
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lf pvdf  (Bio-Rad)
96
Bio-Rad lf pvdf
MiRNAs differentially regulated the allele variants of GWAS lead SNPs. (A–E) Schematic diagram of the miR-153-3p, miR-374b-3p, <t>miR-4274,</t> miR-572 and miR-2964a-5p binding sites on CCDC170 . (F) The overexpression of miR-153-3p significantly suppressed rs6932603-T allele levels in U2OS cells and 293T cells. (G,H) The overexpression of miR-374b-3p and miR-4274 significantly suppressed rs3757322-T allele levels in U2OS cells and 293T cells. (I,J) The overexpression of miR-572 and miR-2964a-5p significantly suppressed rs3748806-G allele levels in U2OS cells and 293T cells. (K) Knockdown of miR-153-3p significantly upregulated rs6932603-T allele levels in U2OS cells and 293T cells. (L,M) Knockdown of miR-374b-3p and miR-4274 significantly upregulated rs3757322-T allele levels in U2OS cells and 293T cells. (N,O) Knockdown of miR-572 and miR-2964a-5p significantly upregulated rs3748806-G allele levels in U2OS cells and 293T cells. (P) Overexpression of these five miRNAs can reduce the expression of CCDC170 at the protein level, while inhibition of this five miRNAs can increase the expression of CCDC170 . The data are from three independent transfection experiments, with assays performed in triplicate ( n =6). Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars show the standard deviation for six technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. ** P <0.01, *** P <0.001.
Lf Pvdf, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+rate+sensor+4274/Trans-Blot+Turbo+RTA+Mini+LF+PVDF+Transfer+Kit/10__1158_slash_0008___5472__can___22___3485-144-38-40
Average 96 stars, based on 1 article reviews
lf pvdf - by Bioz Stars, 2026-11
96/100 stars
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pvdf membranes  (Bio-Rad)
96
Bio-Rad pvdf membranes
MiRNAs differentially regulated the allele variants of GWAS lead SNPs. (A–E) Schematic diagram of the miR-153-3p, miR-374b-3p, <t>miR-4274,</t> miR-572 and miR-2964a-5p binding sites on CCDC170 . (F) The overexpression of miR-153-3p significantly suppressed rs6932603-T allele levels in U2OS cells and 293T cells. (G,H) The overexpression of miR-374b-3p and miR-4274 significantly suppressed rs3757322-T allele levels in U2OS cells and 293T cells. (I,J) The overexpression of miR-572 and miR-2964a-5p significantly suppressed rs3748806-G allele levels in U2OS cells and 293T cells. (K) Knockdown of miR-153-3p significantly upregulated rs6932603-T allele levels in U2OS cells and 293T cells. (L,M) Knockdown of miR-374b-3p and miR-4274 significantly upregulated rs3757322-T allele levels in U2OS cells and 293T cells. (N,O) Knockdown of miR-572 and miR-2964a-5p significantly upregulated rs3748806-G allele levels in U2OS cells and 293T cells. (P) Overexpression of these five miRNAs can reduce the expression of CCDC170 at the protein level, while inhibition of this five miRNAs can increase the expression of CCDC170 . The data are from three independent transfection experiments, with assays performed in triplicate ( n =6). Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars show the standard deviation for six technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. ** P <0.01, *** P <0.001.
Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+rate+sensor+4274/Trans-Blot+Turbo+RTA+Midi+PVDF+Transfer+Kit/pm37468729-309-22-24
Average 96 stars, based on 1 article reviews
pvdf membranes - by Bioz Stars, 2026-11
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Image Search Results


MiRNAs differentially regulated the allele variants of GWAS lead SNPs. (A–E) Schematic diagram of the miR-153-3p, miR-374b-3p, miR-4274, miR-572 and miR-2964a-5p binding sites on CCDC170 . (F) The overexpression of miR-153-3p significantly suppressed rs6932603-T allele levels in U2OS cells and 293T cells. (G,H) The overexpression of miR-374b-3p and miR-4274 significantly suppressed rs3757322-T allele levels in U2OS cells and 293T cells. (I,J) The overexpression of miR-572 and miR-2964a-5p significantly suppressed rs3748806-G allele levels in U2OS cells and 293T cells. (K) Knockdown of miR-153-3p significantly upregulated rs6932603-T allele levels in U2OS cells and 293T cells. (L,M) Knockdown of miR-374b-3p and miR-4274 significantly upregulated rs3757322-T allele levels in U2OS cells and 293T cells. (N,O) Knockdown of miR-572 and miR-2964a-5p significantly upregulated rs3748806-G allele levels in U2OS cells and 293T cells. (P) Overexpression of these five miRNAs can reduce the expression of CCDC170 at the protein level, while inhibition of this five miRNAs can increase the expression of CCDC170 . The data are from three independent transfection experiments, with assays performed in triplicate ( n =6). Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars show the standard deviation for six technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. ** P <0.01, *** P <0.001.

Journal: Biology Open

Article Title: Three functional polymorphisms in CCDC170 were associated with osteoporosis phenotype

doi: 10.1242/bio.050930

Figure Lengend Snippet: MiRNAs differentially regulated the allele variants of GWAS lead SNPs. (A–E) Schematic diagram of the miR-153-3p, miR-374b-3p, miR-4274, miR-572 and miR-2964a-5p binding sites on CCDC170 . (F) The overexpression of miR-153-3p significantly suppressed rs6932603-T allele levels in U2OS cells and 293T cells. (G,H) The overexpression of miR-374b-3p and miR-4274 significantly suppressed rs3757322-T allele levels in U2OS cells and 293T cells. (I,J) The overexpression of miR-572 and miR-2964a-5p significantly suppressed rs3748806-G allele levels in U2OS cells and 293T cells. (K) Knockdown of miR-153-3p significantly upregulated rs6932603-T allele levels in U2OS cells and 293T cells. (L,M) Knockdown of miR-374b-3p and miR-4274 significantly upregulated rs3757322-T allele levels in U2OS cells and 293T cells. (N,O) Knockdown of miR-572 and miR-2964a-5p significantly upregulated rs3748806-G allele levels in U2OS cells and 293T cells. (P) Overexpression of these five miRNAs can reduce the expression of CCDC170 at the protein level, while inhibition of this five miRNAs can increase the expression of CCDC170 . The data are from three independent transfection experiments, with assays performed in triplicate ( n =6). Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars show the standard deviation for six technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. ** P <0.01, *** P <0.001.

Article Snippet: MiR-153-3p mimics, miR-153-3p inhibitors, miR-374b-3p mimics, miR-374b-3p inhibitors, miR-4274 mimics, miR-4274 inhibitors, miR-572 mimics, miR-572 inhibitors, miR-2964a-5p mimics, miR-2964a-5p inhibitors, miRNA NC, inhibitor NC, human CCDC170 siRNA, mouse CCDC170 siRNA, siRNA NC and SNP genotyping oligonucleotide primers were chemically synthesized (Genepharma, Shanghai, China).

Techniques: Binding Assay, Over Expression, Expressing, Inhibition, Transfection, Luciferase, Activity Assay, Standard Deviation, Two Tailed Test

MiRNA overexpression repressed the expression of osteogenesis marker genes and increased the expression of osteoclastogenesis marker genes. (A–C) The overexpression of miR-153-3p, miR-374b-3p and miR-4274 significantly suppressed the expression of Runx2 , Osterix , ALP , Col1a1 , OPN and OCN . (D) The overexpression of miR-572 significantly increased the expression of Runx2 , ALP , Col1a1 , OPN and OCN . (E) The overexpression of miR-2964a-5p significantly suppressed the expression of Osterix , ALP , Col1a1 , OPN and OCN . (F,G,I) The overexpression of miR-153-3p, miR-374b-3p and miR-572 significantly suppressed the expression of WNT4 and WNT16 . (H) The overexpression of miR-4274 significantly suppressed the expression of WNT4 , and the expression level of WNT16 was decreased, but the difference was not significant. (J) The overexpression of miR-2964a-3p significantly suppressed the expression of WNT16 , but the expression level of WNT4 did not change. (K) The overexpression of miR-153-3p significantly suppressed the expression of OPG and significantly increased the expression of TRACP . (L–N) The overexpression of miR-374b-3p, miR-4274 and miR-572 significantly suppressed the expression of OPG and significantly increased the expression of TRACP and CTSK . (O) The overexpression of miR-2964a-5p significantly suppressed the expression of OPG and significantly increased the expression of CTSK . The expression level of TRACP was increased, but the difference was not significant. The data are from three independent transfection experiments, with assays performed in triplicate ( n =8). The error bars show the standard deviation for four technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. NS>0.05, * P <0.05, ** P <0.01, *** P <0.001.

Journal: Biology Open

Article Title: Three functional polymorphisms in CCDC170 were associated with osteoporosis phenotype

doi: 10.1242/bio.050930

Figure Lengend Snippet: MiRNA overexpression repressed the expression of osteogenesis marker genes and increased the expression of osteoclastogenesis marker genes. (A–C) The overexpression of miR-153-3p, miR-374b-3p and miR-4274 significantly suppressed the expression of Runx2 , Osterix , ALP , Col1a1 , OPN and OCN . (D) The overexpression of miR-572 significantly increased the expression of Runx2 , ALP , Col1a1 , OPN and OCN . (E) The overexpression of miR-2964a-5p significantly suppressed the expression of Osterix , ALP , Col1a1 , OPN and OCN . (F,G,I) The overexpression of miR-153-3p, miR-374b-3p and miR-572 significantly suppressed the expression of WNT4 and WNT16 . (H) The overexpression of miR-4274 significantly suppressed the expression of WNT4 , and the expression level of WNT16 was decreased, but the difference was not significant. (J) The overexpression of miR-2964a-3p significantly suppressed the expression of WNT16 , but the expression level of WNT4 did not change. (K) The overexpression of miR-153-3p significantly suppressed the expression of OPG and significantly increased the expression of TRACP . (L–N) The overexpression of miR-374b-3p, miR-4274 and miR-572 significantly suppressed the expression of OPG and significantly increased the expression of TRACP and CTSK . (O) The overexpression of miR-2964a-5p significantly suppressed the expression of OPG and significantly increased the expression of CTSK . The expression level of TRACP was increased, but the difference was not significant. The data are from three independent transfection experiments, with assays performed in triplicate ( n =8). The error bars show the standard deviation for four technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. NS>0.05, * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: MiR-153-3p mimics, miR-153-3p inhibitors, miR-374b-3p mimics, miR-374b-3p inhibitors, miR-4274 mimics, miR-4274 inhibitors, miR-572 mimics, miR-572 inhibitors, miR-2964a-5p mimics, miR-2964a-5p inhibitors, miRNA NC, inhibitor NC, human CCDC170 siRNA, mouse CCDC170 siRNA, siRNA NC and SNP genotyping oligonucleotide primers were chemically synthesized (Genepharma, Shanghai, China).

Techniques: Over Expression, Expressing, Marker, Transfection, Standard Deviation, Two Tailed Test

miRNA knockdown increased the expression of osteogenesis marker genes and repressed the expression of osteoclastogenesis marker genes. (A,B) Knockdown of miR-153-3p and miR-374b-3p significantly increased the expression of Runx2 , Osterix , ALP , Col1a1 , OPN and OCN . (C) Knockdown of miR-4274 significantly increased the expression of Runx2 , ALP , Col1a1 , OPN and OCN . (D) Knockdown of miR-572 significantly suppressed the expression of Runx2 , ALP , Col1a1 and OCN . (E) Knockdown of miR-2964a-5p significantly increased the expression of Osterix , ALP , Col1a1 , OPN and OCN . (F–H,J) Knockdown of miR-153-3p, miR-374b-3p, miR-4274 and miR-2964a-5p significantly increased the expression of WNT4 and WNT16 . (I) Knockdown of miR-572 significantly suppressed the expression of WNT4 and WNT16 . (K,M,O) Knockdown of miR-153-3p, miR-4274 and miR-2964a-5p significantly increased the expression of OPG and significantly suppressed the expression of TRACP and CTSK . (L) Knockdown of miR-374b-3p significantly increased the expression of OPG , significantly suppressed the expression of TRACP , and the expression level of CTSK was suppressed, but the difference was not significant. (N) Knockdown of miR-572 significantly increased the expression of TRACP and CTSK . The error bars show the standard deviation for four technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. NS>0.05, * P <0.05, ** P <0.01, *** P <0.001.

Journal: Biology Open

Article Title: Three functional polymorphisms in CCDC170 were associated with osteoporosis phenotype

doi: 10.1242/bio.050930

Figure Lengend Snippet: miRNA knockdown increased the expression of osteogenesis marker genes and repressed the expression of osteoclastogenesis marker genes. (A,B) Knockdown of miR-153-3p and miR-374b-3p significantly increased the expression of Runx2 , Osterix , ALP , Col1a1 , OPN and OCN . (C) Knockdown of miR-4274 significantly increased the expression of Runx2 , ALP , Col1a1 , OPN and OCN . (D) Knockdown of miR-572 significantly suppressed the expression of Runx2 , ALP , Col1a1 and OCN . (E) Knockdown of miR-2964a-5p significantly increased the expression of Osterix , ALP , Col1a1 , OPN and OCN . (F–H,J) Knockdown of miR-153-3p, miR-374b-3p, miR-4274 and miR-2964a-5p significantly increased the expression of WNT4 and WNT16 . (I) Knockdown of miR-572 significantly suppressed the expression of WNT4 and WNT16 . (K,M,O) Knockdown of miR-153-3p, miR-4274 and miR-2964a-5p significantly increased the expression of OPG and significantly suppressed the expression of TRACP and CTSK . (L) Knockdown of miR-374b-3p significantly increased the expression of OPG , significantly suppressed the expression of TRACP , and the expression level of CTSK was suppressed, but the difference was not significant. (N) Knockdown of miR-572 significantly increased the expression of TRACP and CTSK . The error bars show the standard deviation for four technical replicates of a representative experiment. P -values were calculated using a two-tailed Student's t -test. NS>0.05, * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: MiR-153-3p mimics, miR-153-3p inhibitors, miR-374b-3p mimics, miR-374b-3p inhibitors, miR-4274 mimics, miR-4274 inhibitors, miR-572 mimics, miR-572 inhibitors, miR-2964a-5p mimics, miR-2964a-5p inhibitors, miRNA NC, inhibitor NC, human CCDC170 siRNA, mouse CCDC170 siRNA, siRNA NC and SNP genotyping oligonucleotide primers were chemically synthesized (Genepharma, Shanghai, China).

Techniques: Expressing, Marker, Standard Deviation, Two Tailed Test